ADVANCED INTERACTION SEARCH

VitiSynth Full Entry

Reference: Dynamic translocation of stilbene synthase VpSTS29 from a Chinese wild Vitis species upon UV irradiation
Key Statements

Abstract-

Stilbene phytoalexins derived from grapevine can be rapidly accumulated when exposed to an artificial UV-C treatment. However, the underlying mechanisms involved in this accumulation and translocation are unclear. Here, we describe an investigation of the influence of UV-C treatment on the dynamic subcellular distribution of a member of a stilbene synthase family VpSTS29 derived from Chinese wild Vitis pseudoreticulata W.T. Wang when over-expressed in V. vinifera L. cv. Thompson Seedless. Our results show that VpSTS29-GFP was accumulated at a relatively high level in roots and mature leaves of transgenic grape lines, and was predominantly distributed in the cytoplasm. When exposed to UV-C irradiation, VpSTS29 displayed UV-induced feature coupled with the accumulation of stilbene compounds. Notably, VpSTS29-GFP can be translocated from the cytoplasm into chloroplasts upon UV-irradiation. Leaves from the two VpSTS29-GFP-expressing lines displayed more serious UV damage, showing withering and marginal scorching phenotype, and decreased content of H2O2, compared to the untransformed plant. Also, overexpression of VpSTS29 altered the expression of genes related to redox regulation, stilbene biosynthesis and light stimulus. Co-expression of VpSTS29-GFP with Glycolate oxidase 1 (myc-VpGLO1) confirmed the ability of stilbenes to decrease the content of H2O2 in Arabidopsis mesophyll protoplasts. These results provide new insight into the biological functions and properties of stilbene synthase and its product in response to environmental stimulus.

(highlighting by VitiSynth)

Link to paper-

https://www.sciencedirect.com/science/article/abs/pii/S0031942218308884

https://doi.org/10.1016/j.phytochem.2018.12.019


Partially indexed reference.

View Interactions

VitiSynth Review
Go Back

Vitisynth

Publication Details

Published: 2019
Publication: Phytochemistry
Issue: 2019 159 p137-147
Author: Ma F 2019
Recommendation:

NOT SET

Related Video

None for this Entry

Related Audio

None for this Entry

Discuss this Entry

No related discussions available.

Join Discussion

Latest Discussions
Special Report

Date: 29th October 2015
Reference:Journal:Weed Technology:2015:Online October 2015:Devkota P 2015:Influence of Spray Solution Temperature and Holding Duration on Weed Control with Premixed Glyphosate and Dicamba Formulation

Pesticides in general are chemical products, therefore the quality of ...Read more

Date: 3rd February 2015
Polls:0009:Data standards:03 Malvidin-3-glucoside or Malvidin-3-O-glucoside or Malvidin 3-O-β-D-glucoside?

Malvidin-3-glucoside or Malvidin-3-O-glucoside or Malvidin 3-O-β-D-gl...Read more

Date: 26th January 2015
Admin Synth:Guides:Data standards:Wine industry research papers (draft standard)

I think we should be developing a standard for wine industry research ...Read more

Testimonials.. Read more

test