VitiSynth Full Entry

Reference: Assessment of Tryptophan, Tryptophan Ethylester, and Melatonin Derivatives in Red Wine by SPE-HPLC-FL and SPE-HPLC-MS Methods
Key Statements


Melatonin (MEL) is an indoleamine produced mainly by the pineal gland in vertebrates. It plays a significant role in the regulation of circadian rhythms, mitigation of sleeping disorders, and jet lag. This compound is synthetized from tryptophan (TRP) and it has been found in seeds, fruits, and fermented beverages, including wine. Wine is also a source of other tryptophan derivatives, the tryptophan ethylester (TEE) and MEL isomers (MISs), for which the biological properties need to be elucidated. An analytical method for the simultaneous quantification of TRP, TEE, and MEL was developed by a Solid Phase Extraction (SPE) of a preconcentration of wine followed by high performance liquid chromatography (HPLC) analysis either with fluorescence or mass spectrometer detectors. The analytical method showed a relative standard deviation (RSD) lower than 8%, except for TRP (RSD 10.5% in wine). The recovery was higher than 76%. The versatility of SPE preconcentrations allowed for the adequate preconcentration of wine sample as well as detection of low concentrations, an important aspect especially for MEL (detection limit 0.0023 µg/L). The proposed method proved to be suitable for assessing the investigated compounds in some red wine samples, where 74.4–256.2 µg/L and 0.038–0.063 µg/L of TEE and MEL were detected, respectively. Five MISs were also found in wine samples in concentrations up to 1.97 µg/L.

(highlighting by VitiSynth)

Link to paper-

Partially indexed reference.

View Interactions

VitiSynth Review
Go Back


Publication Details

Published: 2019
Publication: Foods
Issue: 2019 8-3-99
Author: Fracassetti D 2019


Related Video

None for this Entry

Related Audio

None for this Entry

Discuss this Entry

No related discussions available.

Join Discussion

Latest Discussions
Special Report

Date: 29th October 2015
Reference:Journal:Weed Technology:2015:Online October 2015:Devkota P 2015:Influence of Spray Solution Temperature and Holding Duration on Weed Control with Premixed Glyphosate and Dicamba Formulation

Pesticides in general are chemical products, therefore the quality of ...Read more

Date: 3rd February 2015
Polls:0009:Data standards:03 Malvidin-3-glucoside or Malvidin-3-O-glucoside or Malvidin 3-O-β-D-glucoside?

Malvidin-3-glucoside or Malvidin-3-O-glucoside or Malvidin 3-O-β-D-gl...Read more

Date: 26th January 2015
Admin Synth:Guides:Data standards:Wine industry research papers (draft standard)

I think we should be developing a standard for wine industry research ...Read more

Testimonials.. Read more