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Reference: Gimme shelter- three-dimensional architecture of the endoplasmic reticulum, the replication site of grapevine Pinot gris virus
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Grapevine Leaf Mottling and Deformation is a novel grapevine disease that has been associated with grapevine Pinot gris virus (GPGV). The virus was observed exclusively inside membrane-bound structures in the bundle sheath cells of the infected grapevines. As reported widely in the literature, many positive-sense single-stranded RNA viruses modify host-cell membranes to form a variety of deformed organelles, which shelter viral genome replication from host antiviral compounds. Morphologically, the GPGV-associated membranous structures resemble the deformed endoplasmic reticulum described in other virus-host interactions. In this study we investigated the GPGV-induced membranous structures observed in the bundle sheath cells of infected plants. The upregulation of different ER stress-related genes was evidenced by RT-qPCR assays, further confirming the involvement of the ER in grapevine/GPGV interaction. Specific labelling of the membranous structures with an antibody against luminal-binding protein identified them as ER. Double-stranded RNA molecules, which are considered intermediates of viral replication, were localized exclusively in the ER-derived structures and indicated that GPGV exploited this organelle to replicate itself in a shelter niche. Novel analyses using focussed ion-beam scanning electron microscopy (FIB-SEM) were performed in grapevine leaf tissues to detail the three-dimensional organization of the ER-derived structures and their remodelling due to virus replication.

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Due to the complex nature of Ercofid Pure-Liquid and its capability to act on several different wine faults simultaneously, wines can be treated much more precisely and the positive aromas remain unharmed

Publication Details

Published: 2021
Publication: Functional Plant Biology
Issue: 2021 Online July 2021
Author: Pagliari L 2021


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